An ultrasensitive electrochemiluminescent immunosensor based on graphene oxide coupled graphite-like carbon nitride and multiwalled carbon nanotubes-gold for the detection of diclofenac
Liuyi Hu, Jing Zheng, Kang Zhao, Anping Deng*（邓安平）, Jianguo Li*（李建国）
The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, China
Biosensors and Bioelectronics 101(2018) 260--267
In this study, a novel competition-type electrochemiluminescent (ECL) immunosensor for detecting diclofenac (DCF) was fabricated with graphene oxide coupled graphite-like carbon nitride (GO-g-C3N4) as signal probe for the first time. The ECL intensity of carboxylatedg-C3N4was significantly enhanced after being combined with graphene oxide (GO) which exhibited excellent charge-transport property. The sensing platform was constructed by multiwalled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs), which not only provided an effective matrix for immobilizing a large amount of coating antigen but also facilitated the electronic transmission rate to enhance the ECL intensity. Based on the synergistic effect of GO-g-C3N4and MWCNTs-AuNPs composite, the proposed sensor showed high sensitivity, good stability, and wide linearity for the detection of DCF in the range of 0.005–1000 ng mL−1with a detection limit of 1.7 pg mL−1. Furthermore, the developed immunoassay has been applied to real samples with satisfactory results. Therefore, this work provided a promising method for the detection of DCF and other small molecular compounds in the future.
链接：http://www.sciencedirect.com/science/article/pii/S095656631730708X