Multichannel electroanalytical devices for competitive ELISA of phenylethanolamine A
Yang Liua,b, Meng Caib, Weiping Wub, Yuting Fangb, Pei Shea, Shouming Xua, Jianguo Lia,*（李建国）,Kang Zhaoa, Jingjuan Xuc, Ning Baob, Anping Denga,*（邓安平）
a The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, 199 Renai Rd., Suzhou 215123, China
b School of Public Health, Nantong University, 9 Seyuan Rd., Nantong 226019, China
c State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China
Biosensors and Bioelectronics 99 (2018) 21–27
Herein, we reported a disposable electroanalytical device for competitive enzyme-linked immunosorbent assay (ELISA) of phenylethanolamine A (PA). Conductive carbon tape coated with gold cluster served as the working electrode, while the counter and reference electrodes were fabricated on the filter paper by the screen-printing technique. Separating fabrication of the working electrode from other electrodes could make it possible for full utilization of the working electrode modification in bulk and no contamination of the immune-reagents on the counter and reference electrodes. The gold cluster played an important role in both immobilizing antigen and accelerating electron transfer. The eight-channel devices were utilized to detect PA on the strategy of competitive ELISA. The detection range and the limit of detection (LOD) using differential pulse voltammetry for PA were 0.005–60 ng mL−1 and 2.6 pg mL−1, respectively. And also, the linear range for PA performed by square wave voltammetry was 0.05–60 ng mL−1 with the LOD value as 0.028 ng mL−1. The results clearly demonstrated that the proposed electroanalytical devices could be successfully applied in immunoassay and might be further developed for determination of different analytes based on ELISA format.
链接：http://www.sciencedirect.com/science/article/pii/S0956566317302427