Fluorescent nitrogen and sulfur co-doped carbon dots from casein and their applications for sensitive detection of Hg2t and biothiols and cellular imaging
Shouming Xu a, Yang Liu a, b, Hong Yang c, Kang Zhao a, Jianguo Li a, **（李建国）, Anping Deng a, *（邓安平）
a The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China
b School of Public Health, Nantong University, 9 Seyuan Rd., Nantong 226019, China
c College of Pharmacy Sciences, Soochow University, Suzhou 215123, China
Analytica Chimica Acta964 (2017) 150-160
Fluorescent nitrogen and sulfur co-doped carbon dots (NSCDs) were synthesized by a one-step pyrolysis strategy using casein as carbon, nitrogen and sulfur sources, and characterized by UV–vis spectrum, fluorescent spectrum, X-ray photoelectron spectroscopy (XPS) and FT-IR, etc. The synthesized NSCDs displayed a blue emission under ultraviolet illumination with a quantum yield of 31.8%, and a good aqueous solubility, photostability and biocompatibility. It was found that the fluorescence intensity of NSCDs could be selectively quenched by Hg2+, so NSCDs was used as an effective probe for the detection of Hg2+. The linear range and the limit of detection (LOD) of the fluorescent sensor based on NSCDs for the detection of Hg2+ were 0.01–0.25 μM and 6.5 nM, respectively. Spiked water samples were detected by the sensor with the recovery of 95.4–106.3% and relative standard deviation (RSD) of 3.6–8.6%. It was also observed that the quenched NSCDs-Hg2+ system could be restored by the addition of biothiols such as l-cysteine (Lcy), homocysteine (Hcy) and glutathione (GSH), thus NSCDs-Hg2+ system was employed as a fluorescent sensor for the detection of biothiols. The linear range and LOD of the NSCDs-Hg2+ system were 1–10 μM and 23.6 nM for Lcy, 0.2–2.5 μM and 12.3 nM for Hcy, and 0.1–2.0 μM and 16.8 nM for GSH, respectively. The NSCDs-Hg2+ system was applied for the detection of biothiols in serum samples with satisfied results. In addition, the study in vitro imaging HeLa cells revealed that the synthesized NSCDs could be used as effective fluorescent probes in cellular imaging without noticeable cytotoxicity.
链接：http://www.sciencedirect.com/science/article/pii/S0003267017301289