An oxazine-based near-infrared fluorescent probe for selective in cellular imaging of exogenous nitrite
Yan-Hua Zhana, Ru Suna, Wei-Jin Zhua, Yu-Jie Xub, *（许玉杰）, Jian-Feng Gea, *（葛健锋）
aCollege of Chemistry, Chemical Engineering and Material Science, State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, Soochow University, 199 Ren’Ai Road, Suzhou 215123, China
bSchool of Radiation Medicine and Protection, Medicine College of Soochow University, Suzhou 215123, China
Sensors and Actuators B: Chemical,Volume 240, March 2017, Pages 1283–1290
A near-infrared fluorescent probe based on benzo[a]phenoxazine containing o-phenylenediamine group as reactive site was designed and synthesized. The titration experiment showed that the probe gave colorimetric response towards sodium nitrite with the absorption maxima at 630 nm and 542 nm in pH = 5.0 disodium hydrogen phosphate-citric acid buffers containing 30% DMSO; its emission was located in 590–820 nm with the 9.1-fold fluorescent enhancement under excitation at 580 nm. Moreover, the probe can detect nitrite quantitatively in a concentration range of 0.0–26.0 μM and the detection limit is as low as 45 nM. The salient features of this method are high sensitivity and selectivity, fast detection and low detection limit. Fluorescence confocal imaging experiments with V79 and HeLa cells indicate that the probe has the potential application in quantitative detection exogenous nitrite level for in cellular imaging.
链接：http://www.sciencedirect.com/science/article/pii/S0925400516315386