Hemicyanine dyes linked with quaternary ammonium group:Near-infrared probes for the detection of nucleic acid
Xiu-Li Wanga, Ru Suna, Jia-Tao Miaoa, Xin-Sheng Lib,∗（李新生）, Jian-Feng Gea,∗（葛健锋）
aCollege of Chemistry, Chemical Engineering and Material Science, State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials,Soochow University, Suzhou 215123, PR China
bCollege of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321000, PR China
Sensors and Actuators B 236 (2016) 627–634
Two hemicyanine dyes (1a–b) linked with tetraalkyl ammonium group were prepared, and they were evaluated as near-infrared probes for the detection of nucleic acid. Fluorescent responses were observed from 643 to 850 nm when probes 1a–b was titrated with commercially available DNA and RNA. The dimethylaminophenyl containing hemicyanine (1a) gave a 32.8-fold and 33.5-fold emission enhancement towards DNA and RNA, while the dimethylaminonaphthalen containing hemicyanine (1b) gave a 33.0-fold and 38.8-fold emission enhancement respectively. They also show large fluorescence Stokes shifts. The fluorescence of 1a–b distinguishes clear nucleoli staining accompanied by faint cytoplasm in cellular environment, and nucleic acid digest test of ribonuclease indicates their markedly higher affinity for RNA. These results prove that probes 1a–b are very attractive staining reagents for visualizing RNA in fixed cells.
链接：http://www.sciencedirect.com/science/article/pii/S0925400516308619