报告题目：Multiplexing Fluorescence Lifetime Imaging for High Content Screening Applications

报告人：Qiyin Fang, PhD

Associate Professor and Canada Research Chair in Biophotonics

McMaster University

Canada

报告时间：2015年5月20日（星期三）下午 2:00

报告地点：苏州大学独墅湖校区907号楼1445室

报告摘要：

In drug discovery, the ability to quantify protein-protein interactions is crucial in understanding the functioning mechanisms of each drug. Instead of direct visualization of interacting proteins, which well below the diffraction limit, Förster resonant energy transfer (FRET) between fluorophore-tagged proteins can be measured to indicate the degree of interaction at nanometer scale. Measuring FRET through fluorescence lifetime imaging microscopy (FLIM) exploits the intensity independent fluorescence lifetime changes during energy transfer, making it impervious to these intensity-related complications. In high content screening (HCS) of drug leads, throughput is a critical requirement. We developed a FLIM technique, which utilizes lenslet arrays and a streak camera to multiplex the confocal scanning process. This technique achieves high throughput without sacrificing resolution, ideal for HCS. A novel fiber array delay scheme was implemented to reduce crosstalk between adjacent channels, resulting in higher contrast in image reconstructions. The performance of the system in terms of cross-talk, lifetime estimation, and resolution is characterized through live cell imaging experiments.

 
                                                                                                                 (报告联系人：陈红老师课题组)