The application of nitrogen heterocycles in mitochondrial-targeting fluorescent markers with neutral skeletons
Yue Wang a, Bing Xu b, Ru Sun*a（孙如）, Yu-Jie Xub and Jian-Feng Ge *ac（葛健锋）
a College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren’Ai Road, Suzhou 215123, China.
b State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China c Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
J. Mater. Chem. B, 2020, 8, 7466--7474
Four different neutral fluorescent markers containing nitrogen heterocycles (quinoxaline, 1H-pyrazolo[3,4-b]pyridine, 1H-indazole and 1H-pyrrolo[2,3-b]pyridine) as targeting groups were designed and prepared in order to screen out structural units for targeting mitochondria. Several classical fluorophores (coumarin, 1,8-naphthalimide and Nile Red) were connected with these heterocycles via Suzuki coupling reactions. The derivatives of coumarin (dyes 1a and 2a–c) and 1,8-naphthalimide (dyes 3a–c) fluoresced in the blue-green region, while the Nile Red derivatives (dyes 1b and 4a–c) fluoresced in the red light region. The optical properties of the classical fluorophores, such as emission properties and photostability, were retained in the new dyes. All of them showed low cytotoxicity. Confocal fluorescence experiments in L929 normal cells and HeLa cancer cells indicated that dyes 1a–b targeted dual sites of mitochondria and lipid droplets. Moreover, dyes 2a–c, 3a–c and 4a–c targeted mitochondria; meanwhile, there are only a few mitochondria-targeting markers with neutral skeletons. Furthermore, it was found that nitrogen heterocycles with N–H bonds can improve the mitochondrial targeting ability of partial neutral fluorophores.
链接：https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb01377c#!divAbstract