Artificial Channels in an Infectious Biofilm Created by Magnetic Nanoparticles Enhanced Bacterial Killing by Antibiotics
Kecheng Quan a,b, Zexin Zhang a ,*（张泽新）, Hong Chen a, Xiaoxiang Ren b, Yijin Ren c, Brandon W. Peterson b, Henny C. van der Mei, b *, and Henk J. Busscher b ,*
a College of Chemistry Chemical Engineering and Materials Science Soochow University Suzhou 215123, P. R. China
b Department of Biomedical Engineering University of Groningen and University Medical Center Groningen 9713 AV Groningen, The Netherlands
c Department of Orthodontics University of Groningen and University Medical Center Groningen 9713 GZ, Groningen, The Netherlands
Small 2019, 1902313
The poor penetrability of many biofilms contributes to the recalcitrance of infectious biofilms to antimicrobial treatment. Here, a new application for the use of magnetic nanoparticles in nanomedicine to create artificial channels in infectious biofilms to enhance antimicrobial penetration and bacterial killing is proposed. Staphylococcus aureus biofilms are exposed to magnetic-ironoxide nanoparticles (MIONPs), while magnetically forcing MIONP movement through the biofilm. Confocal laser scanning microscopy demonstrates artificial channel digging perpendicular to the substratum surface. Artificial channel digging significantly (4–6-fold) enhances biofilm penetration and bacterial killing efficacy by gentamicin in two S. aureus strains with and without the ability to produce extracellular polymeric substances. Herewith, this work provides a simple, new, and easy way to enhance the eradication of infectious biofilms using MIONPs combined with clinically applied antibiotic therapies.
链接：https://onlinelibrary.wiley.com/doi/pdf/10.1002/smll.201902313