Photothermally Activated Electrospun Nanofiber Mats for HighEfficiency Surface-Mediated Gene Transfection
Yanjun Zheng1, Yong Wu2, Yang Zhou1, Jingxian Wu1, Xiaoyu Wang2, Yangcui Qu1, Yaran Wang1, Yanxia Zhang2*（张燕霞）, and Qian Yu1*（于谦）
1State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China
2 Institute for Cardiovascular Science and Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215007, P. R. China
ACS Appl. Mater. Interfaces 2020, 12, 7, 7905--7914
Although electrospun nanofibers have been used to deliver functional genes into cells attached to the surface of the nanofibers, the controllable release of genes from nanofibers and the subsequent gene transfection with high efficiency remain challenging. Herein, photothermally activated electrospun hybrid nanofibers are developed for high-efficiency surface-mediated gene transfection. Nanofibers with a core–sheath structure are fabricated using coaxial electrospinning. Plasmid DNA (pDNA) encoding basic fibroblast growth factor is encapsulated in the fiber core, and gold nanorods with photothermal properties are embedded in the fiber sheath composed of poly(l-lactic acid) and gelatin. The nanofiber mats show excellent and controllable photothermal response under near-infrared irradiation. The permeability of the nanofibers is thereby enhanced to allow the rapid release of pDNA. In addition, transient holes are formed in the membranes of NIH-3T3 fibroblasts attached to the mat, thus facilitating delivery and transfection with pDNA and leading to increased proliferation and migration of the transfected cells in vitro. This work offers a facile and reliable method for the regulation of cell function and cell behavior via localized gene transfection, showing great potential for application in tissue engineering and cell-based therapy.
链接：https://pubs.acs.org/doi/abs/10.1021/acsami.9b20221