报告题目：Genetically Encoded Protein Chemistry: From Chemical Toolbox to Bioactive Materials

报告人：张文彬特聘研究员（北京大学）

报告时间：2017年5月12日（星期五）下午16:00

报告地点：材料与化学化工学部907幢1549室

报告摘要：

Genetically encoded chemistry provides versatile control over the process of chemical reactions and the resulting materials. The spontaneous formation of an isopeptide bond between a peptide tag and its protein partner is a genetically encoded, cell-compatible, highly specific and efficient chemistry for protein/peptide conjugation, as demonstrated in the chemically reactive pair of SpyTag/SpyCatcher.¹ In this talk, I will give a brief overview of our work in the development of genetically encoded protein chemistry tools and the use of such tools to create bioactive materials. Through protein engineering, we have successfully developed a chemical toolbox of genetically encoded chemical reactions. The possibility to encode chemical information into protein sequences has allowed the direct cellular synthesis of cyclic proteins, tadpole proteins, star proteins, and other branched topologies.² The reaction between proteins bearing multiple reactive groups also lead to all-protein-based bioactive hydrogels, whose macroscopic properties are fully genetically encodable.³ By combining this chemistry with protein folding, protein catenanes and other complex protein topologies can be prepared.⁴ In general, catenation was found to increase proteins’ stability toward proteolytic digestion and thermal denaturation. It has thus opened new ways to engineer protein’s properties, both in vivo and in vitro.

Figure 1. Cellular Synthesis of Protein Catenanes and Mechanically Interlocked Tadpole Proteins through the Use of Genetically Encoded Protein Chemistry

Keywords: Protein Catenanes; SpyTag; SpyCatcher; Isopeptide Bond

1)B. Zakeri, J.O. Fierer, E. Celik, E. C. Chittock, U. Schwarz-Linek, V. T. Moy, M. Howarth, Proc. Natl. Acad. Sci. USA 2012, 109, E690. 2) W. B. Zhang, F. Sun, D. A. Tirrell, F. H. Arnold, J. Am. Chem. Soc.2013, 135, 13988. 3) F. Sun, W. B. Zhang, A. Mahdavi, F. H. Arnold, D. A. Tirrell, Proc. Natl. Acad. Sci. USA 2014, 111, 11269-11274. 4) X. W. Wang, W. B. Zhang, W.-B. Angew. Chem. Int. Ed.2016, 55, 3442-3446.

个人简介：Wen-Bin Zhang

Key Laboratory of Polymer Chemistry & Physics of Ministry of Education,

Center for Soft Matter Science and Engineering,

College of Chemistry and Molecular Engineering, Peking University

Beijing, 100871, China

Phone/Fax: (+86)-10-6276-6876

E-mail: wenbin@pku.edu.cn

URL: http://www.chem.pku.edu.cn/zhangwb/

Research interests:

Giant molecules based on molecular nanoparticles; protein-based biomaterials; catassembly of macromolecules

Biographic description:

Wen-Bin Zhang is currently an Assistant Professor at the Department of Polymer Science and Engineering, College of Chemistry and Molecular Engineering of Peking University. He received his B.S. in Organic Chemistry from Peking University and his Ph.D. in Polymer Science from the University of Akron. He continued at the University of Akron for his postdoctoral research under the supervision of Prof. Stephen Cheng for one year, before he moved to Caltech for a second postdoctoral training with Prof. David Tirrell in Protein Engineering and Biomaterials. His current research interests include the rational development of materials that bridges synthetic systems and biological systems for energy and health-related applications.

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