Dual-Functional Engineered Lactobacillus Rhamnosus for Blocking the Dissemination of Drug Resistance
Shaopeng Huang1, Dinghao Hou1, Yu Rao1, Qingxiang He1, Xiaonan Yuan1, Ziyin Zhao2, Can Jin1, Jiangna Guo(郭江娜)1*, Feng Yan(严锋)1,3*
1Jiangsu Engineering Laboratory of Novel Functional Polymeric Materials, Jiangsu Key Laboratory of Advanced Negative Carbon Technologies, Suzhou Key Laboratory of Soft Material and New Energy, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, China
2Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University,Suzhou, China
3State Key Laboratory of Advanced Fiber Materials, College of Materials Science and Engineering, Donghua University, Shanghai, China
Adv.Funct.Mater., 2026, 36, e77218
Abstract:Drug-resistant pathogens critically endanger lives and threaten global public health. While traditional microbicidal materials can eliminate pathogens, residual drug-resistant genes (DRGs) persist in the environment, potentially spreading resistance via horizontal gene transfer. Consequently, developing new microbiocidal strategies with bacteria and DRGs elimination abilities becomes an urgent priority. Here, we fabricate an integrated living material (LG@PCeO2) by engineering Lactobacillus rhamnosus (LG) with polydopamine (PDA) and in situ grown CeO2 nanoparticles. This bioactive material simultaneously eradicates drug-resistant pathogens and cleaves DRGs, effectively blocking the transmission of resistance. The LG@PCeO2 killed methicillin-resistant Staphylococcus aureus (MRSA) and inhibited/removed corresponding biofilms effectively by synergizing the inherent metabolic properties of LG and the CeO2-enhanced electrostatic effects. Moreover, the PDA coating endows antioxidant capability, while the in situ-grown CeO2 confers DNase-mimicking activity, enabling degradation of extracellular and genomic DRGs for LG@PCeO2. In an MRSA-infected wound model, LG@PCeO2 not only concurrently killed bacteria and eliminated residual DRGs within infected tissue but also inhibited inflammation to effectively block the spread of drug resistance and accelerate wound healing synchronously. This bioactive material offers a promising living-material strategy to combat drug resistance.
Article information: https://doi.org/10.1002/adfm.77218
