Near-Infrared Photoaccelerated MicroRNA Response for Image-Guided Photodynamic-Chemodynamic Synergistic Cancer Therapy via Nanoprobe Programmed Disassembly
Yaojia Zhang1, Jie Sun1, Ling-Hong Xiong2, Ben Zhong Tang3, Xuewen He1(何学文)*
1State Key Laboratory of Bioinspired Interfacial Materials Science, The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China
2School of Public Health Suzhou Medical College of Soochow University, Soochow University, Suzhou 215123, China
3School of Science and Engineering,Shenzhen Institute of Aggregate Science and Technology,The Chinese University of Hong Kong,Shenzhen (CUHK-Shenzhen), Guangdong 518172, China
Adv. Funct. Mater. 2025, 35, 2509090
Abstract:Accurate detection of disease biomarkers in situ is essential for early cancer diagnosis and treatment. Herein, we delicately designed a multifunctional nanoprobe, GNP@Pt-QDs/AIE, for the imaging of miRNA biomarkers in living cells and tissues and image-guided synergistic cancer therapy. Using chimeric phosphodiester-phosphorothioate DNA and dual-thiol grouped aggregation-induced emission (AIE) photosensitizer as co-ligands, monovalent DNA-modified quantum dot (QD) is synthesized with strong photosensitization via efficient energy transfer between QD and the surface-coordinated photosensitizers. Through DNA-programmed assembly with a peroxidase-mimetic platinum-shelled gold nanoparticle (GNP@Pt), a core-satellite GNP@Pt-QDs/AIE nanoprobe is constructed that completely quenched both fluorescence and reactive oxygen species (ROS). Upon response to miRNA target, QDs are programmatically disassembled from the nanoprobes, leading to amplified fluorescence and photodynamic activity. Concurrently, the steric hindrance of the platinum shell is relieved with oxygen-independent chemodynamic activity restored. More significantly, the mild photothermal effect under near-infrared (NIR) irradiation can accelerate the disassembly to fast activate fluorescence and ROS output. Excellent performances in miRNA-21 target imaging and chemodynamic-photodynamic synergistic anticancer therapy are achieved in vitro and in vivo with negligible cytotoxicity. This NIR-photoaccelerable nanoprobe, capable of imaging miRNA biomarkers in situ and synergizing chemodynamic-photodynamic therapy, offers great potential for the early diagnosis and treatment of malignancies.
Article information: https://doi.org/10.1002/adfm.202509090
