报告题目：Functional DNA Nanotechnology: Precise Spatial and Dynamic Control of Nanomaterials Assembly and its Applications in Sensing, Imaging and Medicine
报告人: Yi Lu, Department of Chemistry, Biochemistry, Bioengineering, Materials Science and Engineering, and Beckman Institute for Advanced Science and Technology,
University of Illinois at Urbana-Champaign,
Urbana, IL, 61801
地点: 独墅湖校区907号楼1101报告厅
时间: 2012年3月21日上午10:00-11:30
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报告摘要：
Genetic control of the assembly and disassembly of complex biological structures in response to internal chemical or biological stimuli under ambient conditions have been one of the hallmarks of biology. While proteins play a central role in natural biomaterials, synthetic nanomaterials assembled by nucleic acids are emerging in recent years. DNA has been shown to be highly programmable molecules resulting in a number of 2D and 3D nanostructures. Despite the promise, functionalizing these structures has been challenging. We have developed a novel method of using phosphorothioate DNA as anchors, and a bifunctional linker as a rigid molecular fastener that can connect gold nanoparticles to specific locations on the DNA backbone. Precise distance controls between two nanoparticles or proteins with nanometer resolution have been demonstrated. We are also elucidating DNA “genetic codes” for nanomaterials.
In addition to precise spatial control, dynamic control of the assembly of nanomaterials in response to internal stimuli under ambient conditions is quite important for 3D assembly. To meet this challenge, we took advantage of recent advance in biology, i.e., discovery of functional DNA, a new class of DNAs that can either bind to a target molecule (known as aptamers) or perform catalytic reactions (known as DNAzymes), that are very specific for a wide range of targets, and demonstrated the use of these functional DNA for dynamic control of assembly of gold nanoparticles, iron oxide nanoparticles, quantum dots, and nanotubes, in response to a wide range of chemical and biological stimuli from small metal ions to large biomolecules, including cancer cell markers.1 Because these nanomaterials possess unique optical, electrical, magnetic and catalytic properties, these systems have been converted into colorimetric, fluorescent, electrochemical sensors, and magnetic resonance imaging agents for detection of a broad range of analytes with high sensitivity and selectivity.2 The application of functional DNA nanotechnology has also been expanded to include targeted drug delivery.
报告人简介：
Dr. Yi Lu received his B.S. degree from Peking University in 1986, and Ph.D. degree from University of California at Los Angeles in 1992 under Professor Joan S. Valentine. After two years of postdoctoral research in Professor Harry B. Gray group at the California Institute of Technology, Dr. Lu started his own independent career in the Department of Chemistry at the University of Illinois at Urbana Champaign in 1994. He is now Jay and Ann Schenck Endowed Professor of Chemistry and HHMI Professor in the Departments of Chemistry, Biochemistry, Bioengineering and Materials Science and Engineering. He is also a member of the Center for Biophysics and Computational Biology and Beckman Institute for Advanced Science and Technology. His research interests lie at the interface between chemistry and biology. His group is developing new chemical approaches to provide deeper insight into biological systems. At the same time, they take advantage of recently developed biological tools to advance many areas in chemistry. Specific areas of current interests include a) design and engineering of functional metalloproteins as environmentally benign catalysis in renewable energy generation and pharmaceuticals; b) Fundamental understanding of DNAzymes and their applications in environmental monitoring, medical diagnostics, and targeted drug delivery; and c) Employing principles from biology for directed assembly of nanomaterials and its applications in photonics and sensing. Dr. Lu has received numerous research and teaching awards, including the Fellow of the American Association for the Advancement of Science (2007), Early Career Award, Society of Biological Inorganic Chemistry (2007), Howard Hughes Medical Institute Professor Award (2002), Camile Dreyfus Teacher-Scholar Award (1999), Alfred P. Sloan Research Fellowship (1998), Research Corporation Cottrell Scholars Award (1997), and the Beckman YoungInvestigators Award (1996).
近期论文代表作：
[1] Nandini Nagraj, Yi Lu, "Catalytic Nucleic Acid Biosensors for Environmental Monitoring", in "Nucleic Acid Biosensors for Environmental Pollution Monitoring", Ilaria Palchetti, Marco Mascini, eds.; Royal Society of Chemistry; pp. 82-98 (2011)
[2] Yu Xiang, Yi Lu, "Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets"，Nature Chemistry 3, 697-703 (2011)
[3]Tian Lan, Kimberly Furuya, Yi Lu, "A highly selective lead sensor based on a classic lead DNAzyme", Chemical Communications 46, 3896-3898 (2010)
[4] Juewen Liu, Zehui Cao, Yi Lu, "Functional Nucleic Acid Sensors"，Chemical Reviews 109, 1948-1998 (2009)
[5] Natasha Yeung, Ying-Wu Lin, Yi-Gui Gao, Xuan Zhao, Brandy S. Russell, Lanyu Lei, Kyle D. Miner, Howard Robinson, Yi Lu "Rational design of a structural and functional nitric oxide reductase" Nature 462, 1079--1082 (2009)
[6] Nicholas M. Marshall, Dewain K. Garner, Tiffany D. Wilson, Yi-Gui Gao, Howard Robinson, Mark J. Nilges, Yi Lu, "Rationally tuning the reduction potential of a single cupredoxin beyond the natural range", Nature 462, 113--116 (2009)
[7] Yi Lu, Natasha Yeung, Nathan Sieracki, Nicholas M. Marshall, "Design of functional metalloproteins", Nature 460, 855--862 (2009)
[8] Mehmet Veysel Yigit, Abhijit Mishra, Rong Tong, Jianjun Cheng, Gerard C.L. Wong, Yi Lu, "Inorganic Mercury Detection and Controlled Release of Chelating Agents from Ion-Responsive Liposomes", Chemistry & Biology 16, 937 - 942 (2009)
[9] Yu Xiang, Aijun Tong, Yi Lu, "Abasic Site-Containing DNAzyme and Aptamer for Label-Free Fluorescent Detection of Pb2+ and Adenosine with High Sensitivity, Selectivity, and Tunable Dynamic Range" Journal of the American Chemical Society 131, 15352-15357 (2009)
[10] Daryl P. Wernette, Juewen Liu, Paul W. Bohn, Yi Lu，"Functional DNA-Based Nanoscale Materials and Devices for Sensing Trace Contaminants in Water", MRS Bulletin 33, 34-41 (2008)
应用化学研究所
材料与化学化工学部
2012-3-15
